Abstract The purpose of this study was to investigate the relationship between the acetylcholinesterase (AChE) inhibitory and antigenotoxic effect with the neuroprotective activity of Glaucium corniculatum methanol and water extracts rich in rutin and quercetin flavonoids. Neuroprotective activity in terms of cell survival and development against oxidative damage was measured by MTT assay and microscopic analysis in H2O2-induced NGF-differentiated PC12 (dPC12) cells. QRT-PCR and western blot hybridization method was employed for the determination of AChE inhibition of the extracts in the same cell model, and the genotoxic and antigenotoxic effects were identified with Comet assay with human lymphocytes. H2O2-induced vitality loss in dPC12 cells was inhibited in pre-treated cells with these plant extracts. Moreover, extracts stimulated neurite formation and prevented the oxidative stress-induced reduction in neurite growth. In general, it was determined that G. corniculatum methanol extract containing higher amounts of rutin and quercetin was more effective than water extract in terms of AChE inhibitory, antigenotoxic and also neuroprotective effect. In this study, it was shown for the first time that both AChE inhibitory and antigenotoxic effects of G. corniculatum may be effective in neuroprotection and it’s protective and therapeutic effects against neurodegeneration may be related to the flavonoid content.

Abstract This study aimed to find a pigment of bacterial origin with protective/preventive activity against neurological injury and carcinogenesis, which may be used as a natural food additive in food activity or an active drug ingredient of an anti-neural injury/anticancer agent in the pharmaceutical industry. Within this scope, the Pseudomonas aeruginosa MB713 strain was used to produce pyocyanin. Characterization of the pyocyanin was carried out using UV-VIS, FT-IR, and GC-MS analyses. In addition, its inhibitory action on AChE and antioxidant activities were determined. The cytotoxic activities of the pyocyanin as well as its antiproliferative activities were detected. It was determined that the maximum production of the pyocyanin (51 μg/mL). The pyocyanin did not cause a cell death rate above 50% in L929 cells. The pyocyanin was found to exert an inhibitory effect on the AChE. The pyocyanin was found effective on all antioxidant parameters tested. The IC50 values of the pyocyanin on SK-MEL-30 and HT-29 cells were calculated as 72 and 179 μM, respectively. And due to the pyocyanin’s antioxidant and inhibitory effects on AChE, antiproliferative effects, and protective effects against neurodegenerative injury, the use of the pyocyanin as an active drug ingredient or a preservative food additive may be predicted. This situation can be clarified by conducting advanced molecular studies.

Abstract Among mesenchymal stem cell (MSCs) sources, the most interesting ones in tissue engineering and regenerative medicine are oromaxillofacial tissue. Of these, dental pulp derived (DP-MSCs) MSCs are part of interest. The substantial expansion of MSCs in vitro is a requirement site to achieve adequate cell numbers for cell-based therapy. However, the limited proliferation of MSCs diminishes with long term cell culture amplification. Therefore, natural agents are being investigated to increase proliferation and/or differentiation. Lactobacillus rhamnosus draws attention with the expolysaccharides (EPSs) it produces. EPSs have been attributed to have a significant role in probiotic activity; including immunomodulatory, antitumor, cholesterol lowering, biofilm reducing, antioxidant, antiallergic, and wound healing effects. However, there is incompetent knowledge discussing their effect on DP-MSCs. In this study we aimed to demonstrate the induced proliferation, differentiation and cellular collagen secretion of DP-MSCs in response to the L. rhamnosus E9 EPSs. 1000 μg/mL EPSs was determined as the effective concentration by using a real-time monitoring system. L. rhamnosus E9 EPS was able to accelarate ostegenic differentiation and secretion of cellular collagen. Indeed, EPS was able to decreased the calcium granules that occurs due to long term cultivation. By consideration of inducing proliferation, osteogenic differentiation and secretion of cellular collagen, L. rhamnosus EPS could be considered as good candidate for preconditioning agent and/or scaffold material.

Exopolysaccharides (EPSs) production was studied by Lactobacillus delbrueckii subsp. bulgaricus (B3, G12) and Streptococcus thermophilus (W22) in the medium containing various carbon sources (glucose, fructose, sucrose or lactose). For all the strains, glucose was the most efficient carbon source and B3, G12 and W22 strains produced 211, 175 and 120 EPS mg/L respectively. Also, the influence of different concentrations of glucose (5,10,15,20,25,30 g/L) on EPS production and growth was studied. The results indicated that EPS production and growth were stimulated by the high glucose concentration (30 g/L).